Biotin anticorps (Rhodamine)

N° du produit ABIN401178

Cet anticorps anti-Biotin est un anticorps Lapin Polyclonal détectant Biotin dans FACS et IF. Adapté pour .

Aperçu rapide pour Biotin anticorps (Rhodamine) (ABIN401178)

Antigène: Biotin

Reactivité: Veuillez nous consulter SVP

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Biotin est conjugé à/à la Rhodamine

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Détail du produit anti-Biotin anticorps (Rhodamine)

Attributs du produit: Absorption / Emission: 550 nm / 570 nm
Molar Ratio: 5.0 Moles TRITC per mole of Goat IgG

Purification: Affinity chromatography

Immunogène: Biotin conjugated to Keyhole Limpet Hemocyanin (b-KLH)

Isotype: IgG

Information d'application

Indications d'application: Immunofluorescence: 1/500 - 1/2,500. Flow Cytometry: 1/2,000 - 1/10,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur Biotin

Antigène: Biotin

Classe de substances: Chemical

Sujet: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.